Are you buying Hepatitis b virus surface antigen(HBsAg) quantitative detection kit (immunofluorescence method)?

Hepatitis B virus (HBV) expresses three types of surface antigens, i.e. S-, M-, and L-protein. L-protein is composed of S-, Pre-S2, and Pre-S1 region. The deletion of Pre-S1 region forms M-protein, and further deletion of Pre-S2 region results in S-protein. Most of commercially available HBsAgs is composed of either S-protein alone or S- plus M-proteins. This product, HBsAg, L-protein contains all the three components in one protein.
Characteristics: This immunoassay kit allows for the in vitro quantitative determination of human HBsAg concentrations in serum, plasma and other biological fluids.
Application Notes    
Centrifuge vials before opening to collect contents. When mixing or reconstituting protein solutions, always avoid foaming. To avoid cross-contamination, change pipette tips between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent. When using an automated plate washer, adding a 30 second soak period following the addition of wash buffer, and/or rotating the plate 180 degrees between wash steps may improve assay precision. To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Substrate Solution should remain colorless until added to the plate. Keep Substrate Solution protected from light. Substrate Solution should change from colorless to gradations of blue. 8 Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution. Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.
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